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<Title>XY: Gaddum/Schild EC50 shift (nonlin. reg.)</Title>
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<WebLink Flags="0" ToolTip="@help:REG_GaddumSchild" URL="@help:REG_GaddumSchild">
Step by step instructions
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<B><Font Size="11" Face="Verdana">
How the data are organized
</Font></B>
<Font Size="11" Face="Verdana">
<BR/>The X values are the logarithm of the concentration of agonist. The values in column A are the response of agonist with no antagonist present. The values in column B are the response in the presence of 0.3 nM of antagonist. Columns C, D and E are responses in the presence of other concentrations of antagonist, shown in the column titles. 
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<BR/><BR/>The goal
</Font></B>
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<BR/>To fit the Gaddum/Schild model of competitive antagonism to determine the pA2, the negative log of the concentration that shifts the dose-response curve by a factor of 2.0.
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<B><Font Size="11" Face="Verdana">
<BR/><BR/>How to fit the data
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<BR/>1. Click Analyze, and choose Nonlinear regression from the list of XY analyses.
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<BR/>2. Choose the group of equations: Dose-response - Special.
</Font>
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<BR/>3. Choose the Gaddum/Schild EC50 shift model. 
</Font>
<Font Size="11" Face="Verdana">
<BR/>4. View the results, including the pA2 value, and view the graph.
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<WebLink Flags="0" ToolTip="@help:reg_how_global_nonlinear_regressio" URL="@help:reg_how_global_nonlinear_regressio">
Learn about global nonlinear regression
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Global nonlinear regression
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<BR/>This example demonstrates the usefulness of  global nonlinear regression. The parameters of interest cannot be determined by fitting any data set alone, but can be determined by globally fitting all data sets at once.
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<Title>3e-10</Title>
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