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<Title>XY: Operational model (nonlin. reg.)</Title>
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<WebLink Flags="0" ToolTip="@help:REG_Operational_model_depletion" URL="@help:REG_Operational_model_depletion">
Learn more about the operational model
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<B><Font Size="11" Face="Verdana">
How the data are arranged
</Font></B>
<Font Size="11" Face="Verdana">
<BR/>The X values are the logarithm of the concentration of agonist. Column A has response without pretreatment. Column B is response after pretreating to reduce receptor number.
</Font>
<B><Font Size="11" Face="Verdana">
<BR/><BR/>The goal
</Font></B>
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<BR/>To fit the operational model to determine the Ka of binding of the agonist to the receptors, a value that can be very different than the EC50.
</Font>
<B><Font Size="11" Face="Verdana">
<BR/><BR/>How to fit the data
</Font></B>
<Font Size="11" Face="Verdana">
<BR/>Click Analyze, choose nonlinear regression, and choose the panel of equations:  
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<I><Font Size="11" Face="Verdana">
Dose-response --  Special. 
</Font></I>
<Font Size="11" Face="Verdana">
Choose the equation: 
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<I><Font Size="11" Face="Verdana">
Operational model -- Receptor depletion.
</Font></I>
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<WebLink Flags="0" ToolTip="@help:reg_how_global_nonlinear_regressio" URL="@help:reg_how_global_nonlinear_regressio">
Learn about global nonlinear regression
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Global nonlinear regression
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<BR/>This example demonstrates the usefulness of  global nonlinear regression. The parameters of interest cannot be determined by fitting either data set alone, but can be determined by globally fitting both data sets at once.
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<Title>log [agonist]</Title>
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<d>-8</d>
<d>-7</d>
<d>-6,5</d>
<d>-6</d>
<d>-5,5</d>
<d>-5</d>
<d>-4,5</d>
<d>-4</d>
<d>-3,5</d>
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<Title>Control</Title>
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<d>516</d>
<d>950</d>
<d>2863</d>
<d>7920</d>
<d>11777</d>
<d>14437</d>
<d>14627</d>
<d>14701</d>
<d>15860</d>
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<Title>Receptor depletion</Title>
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<d>241</d>
<d>423</d>
<d>121</d>
<d>527</d>
<d>745</d>
<d>3257</d>
<d>3815</d>
<d>4984</d>
<d>5130</d>
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